Cod proiect: PN-III-P1-1.1-TE-2019-1506
Numar contract: 147/2020
Perioada de implementare: 01/11/2020 – 01/10/2022.
Titlu proiect: Interactiuni in vitro intre nanoparticule si tulpini bacteriene cu potential probiotic izolate din microbiota
Project title: In vitro interactions of nanoparticles with human microbiota and probiotic strains
Acronim: Nano-microbiota
Rezumat (in limba romana): O mare parte din cercetările științifice recente susțin ideea că terapiile bazate pe modularea microbiotei pot fi utilizate ca metodă de control a metabolismului gazdei și în consecință, de evitare a dezvoltării multor boli, cum ar fi sindromul metabolic, obezitatea, bolile inflamatorii intestinale, bolile hepatice, bolile infecțioase emergente, diabet, ateroscleroză, cancer, alergii și afecțiuni ale sistemului nervos central (depresia, anxietatea, boala Parkinson, boala Alzheimer, scleroza multiplă și autism). De la produsele alimentare de baza, la utilizarea de produse cosmetice si farmaceutice care pot fi ingerate frecvent, pana la utilizarea de sisteme nanostructurate pentru administrarea țintită a unor medicamente, microbiota rezidentă este expusa in permanenta actiunii nanoparticulelor (NPs). Efectul NPs asupra membrilor microbiotei normale (situata in cea mai mare parte la nivel gastro-intestinal) sunt foarte putin cunoscute. Scopul acestui proiect este de a investiga interacțiunea in vitro dintre NPs anorganice, intalnite pe scară largă în practicile medicale comune, și tulpini bacteriene cultivabile cu potential probiotic izolate din microbiota. Stabilirea impactului unor astfel de nanomateriale, cu privire la proprietățile antimicrobiene potentiale ale acestora (respectiv inhibarea creșterii și multiplicării, modularea aderenței microbiene și controlul fenomenului de rezistență la antibiotice) asupra microbiotei și/sau asupra speciilor cu potențial probiotic, ar îmbunătăți semnificativ cunoștințele actuale cu privire la modularea echilibrului microbiotei normale in societatea moderna. Deoarece microbiota are capacitatea de a controla numeroase boli moderne, cunoasterea impactului NPs asupra membrilor microbiotei este esentiala pentru instalarea si progresia a numeroase boli (in special a infectiilor).
Abstract (english): A large amount of recent scientific evidence supports the idea that microbiota-based therapies can be used to modulate host metabolism and the development of numerous diseases, such as metabolic syndrome, obesity, inflammatory bowel disease, liver diseases, emerging infectious diseases, diabetus mellitus, atherosclerosis, cancer, allergies, and central nervous system disorders, including depression, anxiety, Parkinson’s disease, Alzheimer’s disease, multiple sclerosis and symptomologies of autism spectrum disorder. Such approaches are expected to move from bench to bedside in the near future. From ingested food, to the use of daily products that can be easily ingested, and also to the use of nanosized drug delivery systems for therapy, resident microbiota is prone to be “influenced” by these nanomaterials in widely undiscovered ways. The aim of this project is to investigate the in vitro interactions among inorganic nanoparticles (NPs) widely encountered in common practices and cultivable microbiota and probiotic isolates. Establishing the impact of such nanomaterials, in terms of antimicrobial properties, growth inhibition and antipathogenic protection properties (i.e. modulation of the microbial attachment, pathogen growth inhibition, antibiotic resistance control etc) on model microbiota with probiotic potential species would significantly improve the current knowledge regarding microbiota modulation. Since the microbiota has the ability to control the progress of numerous modern diseases, the impact of such nanosized materials in their development and clinical outcome via microbiota modulation (especially infection control) could be significant.
Echipa proiectului:
Alina Maria Holban – Director
Lia Mara Ditu – Cercetator
Alexandru Mihai Grumezescu – Cercetator
Irina Gheorghe – Cercetator postdoctoral
Gratiela Gradisteanu – Cercetator postdoctoral
Ecaterina Sarbu – Doctorand
Alexandra Burdusel – Doctorand
Articole ISI:
1. Mohammed, H.B.; Rayyif, S.M.I.; Curutiu, C.; Birca, A.C.; Oprea, O.-C.; Grumezescu, A.M.; Ditu, L.-M.; Gheorghe, I.; Chifiriuc, M.C.; Mihaescu, G.; Holban, A.-M. Eugenol-Functionalized Magnetite Nanoparticles Modulate Virulence and Persistence in Pseudomonas aeruginosa Clinical Strains. Molecules 2021, 26, 2189. https://doi.org/10.3390/molecules26082189
2. Gherasim, O.; Popescu, R.C.; Grumezescu, V.; Mogoșanu, G.D.; Mogoantă, L.; Iordache, F.; Holban, A.M.; Vasile, B.Ș.; Bîrcă, A.C.; Oprea, O.-C.; Grumezescu, A.M.; Andronescu, E. MAPLE Coatings Embedded with Essential Oil-Conjugated Magnetite for Anti-Biofilm Applications. Materials 2021, 14, 1612. https://doi.org/10.3390/ma14071612
3. Chircov, C.; Ștefan, R.-E.; Dolete, G.; Andrei, A.; Holban, A.M.; Oprea, O.-C.; Vasile, B.S.; Neacșu, I.A.; Tihăuan, B. Dextran-Coated Iron Oxide Nanoparticles Loaded with Curcumin for Antimicrobial Therapies. Pharmaceutics 2022, 14, 1057. https://doi.org/10.3390/pharmaceutics14051057
4. Lazar V, Holban AM, Curutiu C, Ditu LM. Modulation of Gut Microbiota by Essential Oils and Inorganic Nanoparticles: Impact in Nutrition and Health. Front Nutr. 2022 Jul 8; 9:920413. doi: 10.3389/fnut.2022.920413.
Lucrari prezentate la conferinte internationale:
1. A.M. Holban, B.A. Iacob, D. Iordache, A.M. Grumezescu, F. Marinescu, L.M. Ditu, C. Curutiu, V. Lazar. Inorganic nanoparticles modulate antipathogenic effects of microbiota isolates with impact on the infectious process. 31st European Congress of Clinical Microbiology & Infectious Diseases (ECCMID), 2021, Austria, Viena, 09 – 12. 07. 2021.
2. A.M. Holban, A. Burdusel, A.M. Grumezescu, L.M. Ditu, C. Curutiu, I. Gheorghe, V. Lazar. Effect of inorganic nanoparticles on probiotic and microbiota isolated bacteria strains. EMRS Spring Meeting, Strasbourg, Franta, 31.05. – 04.06. 2021.
3. Holban, A.M., Grumezescu, A.M., Capraru, P., Asaftei, M., Ditu, L.M., Curutiu, C., Puscas, N., Lazar, V. Interference of oxide nanoparticles with microbiota isolated Enterococcus sp strains. EMRS 2022, Strasbourg, Franta, online, 30.05 – 03.06.2022.
4. Holban AM, Grumezescu AM, Ditu LM, Curutiu C, Grumezescu V, Van Luc T, Lazar V, Gheorghe I. Inorganic nanoparticles functionalized with Eugenol to Modulate Virulence in Pseudomonas aeruginosa Clinical isolates. 32nd European Congress of Clinical Microbiology & Infectious Diseases; Portugalia, Lisabona, 22. – 27.04.2022.
5. Holban, A.M., Grumezescu, A.M., Ditu, L.M, Curutiu, C., Lazar, V., Chifiriuc M.C. Inorganic nanoparticles alter growth and antipathogenic effects of probiotic Lactobacillus sp. strains. FEMS 2022; Belgrad, Serbia, 30.06 – 02.07.2022.